Following the Field Science Course will be the Advanced Laboratory Skills in Field Biology. If you are planning to take both the Field Science and Advanced Laboratory Skills in Field Biologys, we will be using samples collected from the Field Science Course in Yakushima Island for the Advanced Laboratory Skills in Field Biology. Therefore, we recommend that you take a Advanced Laboratory Skills in Field Biology accordingly to the Field Science course you took (if you take the Field Science Course). In addition, if you do not have experience with genome experiments, we recommend Advanced Laboratory Skills in Field Biologys A) - C). These combinations enable you to analyze the samples collected by yourselves. If you are interested in NGS and data analysis for NGS, take course D), regardless of the group you took in the Field Science Course (if you did take any Field Science Courses).
A) Individual identification of fecal DNA
We will focus on DNA individual identification method based on genotyping. DNA of monkeys and deer will be extracted from the feces collected during the Field Science Course. After DNA extraction, we will identify the sex using genetic marker and will identify individuals using several microsatellite loci.
B) Insect DNA barcoding
The Insect group will collect and identify insect species in Yakushima. Using these collected insects, we will sequence the mitochondrial DNA in order to provide a DNA barcode catalogue of insects in Yakushima. Based on the DNA barcode catalogue thus obtained, we will analyze the food repertories of Yakushima monkeys using the monkey feces-derived sequence data which were obtained by the members of 2014 Genome Training Course using MiSeq next generation sequencer.
C) Mushroom DNA barcoding
The Mushroom DNA group will collect and identify mushroom species in Yakushima. Using these collected mushrooms, we will sequence the ITS region of the genomic DNA in order to provide a DNA barcode catalogue of mushrooms in Yakushima Island.
D) Whole genome analyses of the Yakushima monkey
Lecturers will provide a whole genome shotgun (WGS) sequence of a Yakushima monkey, Macaca fuscata yakui
determined by HiSeq2000 high-throughput sequencer. Using this data, we will try to analyze the functional gene sequences (e.g.
, sensory receptor gene repertoire), genetic diversity in the genome-wide scale, genetic differences with other macaque species, and historical changes of the effective population sizes of Yakushima monkeys in order to reveal the ecology and evolution of Yakushima monkeys. This group will not do "wet" laboratory experiments, but will focus on "dry" bioinformatics using personal- and super- computers.
Date: June 9 (Tue), 2015
Place: Science Seminar House, Yoshida Campus of Kyoto University.
We will have talks from visiting foreign students and host researchers. We will also present the results of Field Science Course and Advanced Laboratory Skills in Field Biology in the poster session.